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清华学者最新Nat Biotech论文,开发出基因编辑脱靶检测技术
图2. Tracking-seq检测不同编辑工具的链特异性信号 得益于Tracking-seq的高泛用性,本研究发现当使用相同的向导RNA时,Cas9、CBE、ABE和PE的脱靶效应存在明显差异,其中Cas9和CBE之间观测到了许多近似于各自特异的脱靶位点。
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