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Neurogenesis within the adult central nervous system is demonstrated using an exogenous cell tracer, 5'-bromo-2'-deoxyuridine (BrdU), in combination with endogenous neuronal markers. Specific ...
Cell proliferation was determined by a BrdU assay (Roche Applied Science, Indianapolis, IN, USA) measuring the incorporation of the pyrimidine analogue, 5-bromo-2′-deoxyuridine (BrdU) during DNA ...
BrdU in single stranded DNA, free BrdU or BrdU coupled to protein carrier. S-phase of the cell cycle. Proliferation marker. Weak cross with chloro deoxyuridine but not thymidine. Comments / Questions.
Assays to assess the proliferative activity of cells grown in culture or harvested from tissue samples are a core tool for monitoring the health and growth rate of a cell population.
Each of these protocols is described in great detail, making this paper an excellent resource for scientists who want to attempt an assay to assess DNA damage. * Abstract from Nature Protocols ...
Each assay has specific advantages and disadvantages. For example, BrdU assays, a common DNA synthesis cell proliferation analysis method, has the advantage of single-cell resolution, but can ...
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