Neurogenesis within the adult central nervous system is demonstrated using an exogenous cell tracer, 5'-bromo-2'-deoxyuridine (BrdU), in combination with endogenous neuronal markers. Specific ...

Cell proliferation was determined by a BrdU assay (Roche Applied Science, Indianapolis, IN, USA) measuring the incorporation of the pyrimidine analogue, 5-bromo-2′-deoxyuridine (BrdU) during DNA ...

BrdU in single stranded DNA, free BrdU or BrdU coupled to protein carrier. S-phase of the cell cycle. Proliferation marker. Weak cross with chloro deoxyuridine but not thymidine. Comments / Questions.

Assays to assess the proliferative activity of cells grown in culture or harvested from tissue samples are a core tool for monitoring the health and growth rate of a cell population.

Each assay has specific advantages and disadvantages. For example, BrdU assays, a common DNA synthesis cell proliferation analysis method, has the advantage of single-cell resolution, but can ...

Each of these protocols is described in great detail, making this paper an excellent resource for scientists who want to attempt an assay to assess DNA damage. * Abstract from Nature Protocols ...