3| Add 1 μl of 0.25 M EDTA to the small-scale reaction containing the radioisotope and transfer it to ice. Heat the large-scale reaction to 70 °C for 10 min and transfer it to ice. 4| Measure ...

Dilute the dA-tailed cDNA to a final volume of 1 ml per reaction with Tris-EDTA buffer (pH 7.6). The tailing reaction can be optimized by setting up mock reactions containing ∼50 ng of a control ...