Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a ...

In vivo protein expression includes cloning a gene of interest with the essential control elements into an expression vector, and then transforming the vector into an easily grown organism or cell ...

A universal vector (IL-60 and auxiliary constructs), expressing or silencing genes in every plant tested to date, is described. Plants that have been successfully manipulated by the IL-60 system ...

Escherichia coli is widely considered the most cost-effective bacterial system for recombinant protein expression. Consequently, it is broadly used. Nonetheless, it has its limitations as an ...

Recombinant protein expression is an essential pillar of biomedical research. Efforts from enzyme discovery to protein structure-function study to development of drugs and biological therapeutics all ...