Immobilized metal affinity chromatography (IMAC) is a reliable method for isolation of recombinant protein following over-expression in Escherichia coli. The polyhistidine tag (6–10 consecutive amino ...

Vortex fluidic devices (VFDs) used with immobilized metal affinity chromatography (IMAC) have provided an effective, albeit cumbersome, way to separate His-tagged proteins. Researchers recently ...