本研究针对生物药糖基化分析通量低、定量准确性不足的瓶颈，开发了基于MALDI-TOF-MS的全糖组内标法。通过优化96孔板兼容的Sepharose CL-4B纯化流程，实现了单次实验192样本的高精度分析（CV~10%）。该方法在曲妥珠单抗和EPO等复杂糖蛋白的质控中展现出优异线性（R2 ...

本文通过MALDI-TOF质谱系统研究了星形与线性聚合物（PLA、PEO）在混合体系中的信号强度差异，发现星形聚合物因拓扑结构（更多配位位点、更高氧原子密度）表现出显著增强的离子化效率。结合DFT计算验证了星形聚合物与钾离子（K+）结合能更高，为聚合物定量 ...

Homology surveillance of carbapenem-resistant Klebsiella pneumoniae (CRKP) is critical to monitor and prevent outbreaks of nosocomial infections. In the present study, a matrix-assisted laser ...

What is time-of-flight mass spectrometry? How ToF MS has gained more interest since the commercialization of MALDI. Why a high-speed ADC is key to ToF MS performance. Mixed-signal front end that ...

The inimitable adaptability of MALDI-TOF/TOF systems enables tissue imaging of intact proteins, as well as of tryptic peptides brought about by on-tissue digestion. Although intact protein imaging can ...

MALDI imaging — an analytical technique that detects potential biomarkers directly from tissue sections — has become popular over the last 10 years. One significant aspect is the development protocols ...

Boston-based PerkinElmer Life and Analytical Sciences has released the prOTOF™ 2000, the first commercially available matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass ...

Recently developed Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) requires innovative methods for designing automated liquid handling systems. An overview ...

EGFR mutation and copy number, EGFR protein expression and KRAS mutation as predictors of outcome with erlotinib in bronchioloalveolar cell carcinoma (BAC): Results of a prospective phase II trial ...