All peripheral blood or bone marrow samples in this study were derived from CML patients admitted to the MD Anderson Cancer Center from January 1997 to April 2002. Where indicated, only those patient ...

这篇研究通过创新的巢式PCR（nested PCR）两步扩增策略，显著提升了rpoB基因标记在低浓度细菌DNA和宿主相关微生物组（host-associated microbiota）宏条形码（metabarcoding）分析中的灵敏度。该方法克服了传统单步PCR因引物高度简并性导致的扩增效率低下问题，通过体外 ...

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale ...