本研究通过头对头比较Vircell、Certest和Altona三种HDV RNA qRT-PCR检测试剂盒,揭示了其在定性诊断上高度一致(κ=0.988-1.000),但存在显著的定量偏倚(Vircell和Certest分别高估0.33和0.24 log10IU/mL)。同时,研究证实热休克(thermal shock)前处理虽能提升低病毒载量样本的 ...
Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of ...
In a very short time, qRT-PCR has emerged as one of the most useful approaches in a scientist’s toolbox. Advances in instrument capabilities and the many types of reagents now available are driving ...
(A, B) The qRT-PCR results showed increased mRNA expression of HBEGF, EREG, and AREG induced by Yoda1 in endothelial cells and was reversed with Piezo1 knockdown in HUVEC cells. (C, D) the ELISA assay ...
Breast cancer in a patient with multiple endocrine neoplasia type 1 (MEN 1): A case report and review of the literature. This is an ASCO Meeting Abstract from the 2012 ASCO Annual Meeting I. This ...