The clinical course of neuroblastoma (NB) varies greatly in individual children depending on age, stage, histology and a number of genetic features (Brodeur, 2003; Maris et al, 2007). It is ...
qRT-PCR是检测低拷贝RNA的一种比较实用的技术。qRT-PCR的应用包括在感染性疾病研究中定量病毒RNA,分析非编码RNA如miRNA或lncRNA,研究临床样品如甲醛固定、石蜡包埋(FFPE)样品的检测。低拷贝数的转录物,如编码转录因子的mRNA,是某些细胞和组织类型具有固有的 ...
Although qRT-PCR studies provide valuable information about the relative expression of transcripts in a cell, they are difficult to perform with very small numbers of cells (for example, 1–1,000 cells ...