On average, the DNA yield per sample was (specific range) ng/μL, with high extraction efficiency observed across all samples. Sufficient DNA was obtained for downstream 16S rRNA sequencing. 2.3 ...

Researchers analyzed 168,464 samples to uncover how geographic and technical factors shape global gut microbiome variation, ...

A study revealed that maternal allergies and the season of an infant’s birth influence the breast milk microbiome, impacting ...

Duodenal biopsies were obtained aseptically, and the mucosa-associated microbiota (MAM) analysed via 16S rRNA gene amplicon sequencing. Conclusion This study suggests a link between the duodenal MAM, ...

DNA sequencing is any chemical, enzymatic or technological procedure for determining the linear order of nucleotide bases in DNA. Sanger sequencing by replicative synthesis in the presence of ...

DNA sequencing is the process used for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA. DNA sequencers are scientific instruments ...

In the present study, sequencing of four typical Xiaoqu was performed using the Illumina MiSeq ... The V3–V4 hypervariable regions of the 16S rRNA genes were amplified by universal primers (F: 5′- ...

Finally, a sequence comparison of RlmI orthologues among bacteria indicated that some important human pathogens are conserved in the c-di-GMP-based rRNA regulatory mechanism. To more accurately ...

Abstract: Accurate cell classification is crucial but expensive for large-scale single-cell RNA sequencing (scRNA-seq) analysis. Gene selection (GS) emerges as a pivotal technique in identifying gene ...

Jan. 30, 2025 — A research group has developed new advanced light-controlled tools that enable precise control of proteins in real time in living cells. This groundbreaking research opens doors ...