For determination of protein concentration, Pierce bicinchoninic acid assay (BCA, Thermo Scientific, 23225) (Smith et al, 1985) was performed in microplate format according to manufacturer’s ...

Extracellular vesicles have emerged as promising tools in regenerative medicine due to their inherent ability to facilitate intercellular communication and modulate cellular functions. These nanosized ...

The Food and Drug Administration (FDA, the Agency, or we) is classifying the herpes simplex virus nucleic acid-based assay for central nervous system infections into class II (special controls). The ...

The resulting lysate was normalized with a bicinchoninic acid (BCA) protein assay kit (Pierce, 23225) to contain equal amounts of total protein. Samples were denatured in 2.4× Laemmli buffer with 10 ...

The protein concentration of the cell lysates was assessed using a bicinchoninic acid assay. For western blots, we used primary antibodies against fibronectin (Abcam, ab2413), periostin (Abcam, ...

Subsequently, 50 μL of elution buffer containing 0.1 M formic acid was added to release the IgGs. To neutralize the mixture, 50 μL of neutralization buffer was added until the mixture reached a ...

Cellular respiration was evaluated using the Cell Mito Stress Test (Agilent, 103015-100) in triplicate, following the manufacturer’s instructions. OCR data were normalized to cell number per well ...

Targeting cancer-associated fibroblast metabolism or histone lactylation in pancreatic cancer cells to reverse epigenetic remodeling induced by lactate accumulation in the tumor microenvironment are ...