Briefly, proteins were extracted by RIPA buffer (Vazyme Biotech Co., Ltd.) and quantitated with a bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific, Inc.). Subsequently, 30 µg proteins were ...

Subsequently, the samples underwent concentration and buffer exchange, and the protein content was quantified using the bicinchoninic acid assay method, in accordance with the instructions provided in ...

The quantitative end-point chromogenic tachypieus amebocyte lysate assay kit (EC80545S) was purchased from Xiamen Houshiji (Xiamen, China). ZO-1 antibody (21773-1-AP) and occludin antibody (66378-1-lg ...