Protein concentrations were quantified using a bicinchoninic acid assay (BCA) (Pierce Biotechnology, Waltham, MA). Five micrograms of protein [1X Laemmli sample buffer (Bio-Rad Laboratories), 0.1M DTT ...
Glycolic acid is a substance that chemically exfoliates a person’s skin by dissolving dead skin cells and oils. Glycolic acid may also help boost collagen production, and support skin moisture.
Protein concentration was determined using the bicinchoninic acid assay. Isolation of sulfane sulfur-binding protein was performed as described in the Experimental procedures. Sulfane sulfur ...
Subsequently, the samples underwent concentration and buffer exchange, and the protein content was quantified using the bicinchoninic acid assay method, in accordance with the instructions provided in ...
Another common use of colorimetric assays includes total protein quantification. For example, the BCA assay is one that combines the reduction of Cu2+ into Cu1+ by proteins in an alkaline medium, with ...
The worms were collected on day 6 of the PA intervention. Protein was extracted by homogenization using a sonicator, and protein concentrations were determined by a Bicinchoninic Acid Protein Assay ...
Folate and folic acid are two forms of vitamin B9. Folate refers to the natural form of vitamin B9 found in foods, like leafy green vegetables. Folic acid is the synthetic (man-made) version found in ...
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