Ubiquitin-binding domains (UBDs) are modular elements that bind non-covalently to the protein modifier ubiquitin. Specific ubiquitin–UBD interactions are crucial for the regulation of multiple ...
In this webinar brought to you by Lonza, Ethan Shelkey and Aurita Menezes will discuss the effects of donor variability in cell-based assays for measuring drug immunogenicity and immunotoxicity.
1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an ...