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After 24 h, transfected cells were submitted to a QCM ECMatrix Cell Invasion Assay and a MTT Cell Growth Assay (Millipore) for 48 h. Colorimetric reading of the Invasion Assay was done at OD560 nm; ...
Ago2/CAV1 interaction plays a role in miRNA-mediated mRNA suppression and in miRNA release via extracellular vesicles (EVs) from tumors into the circulation, which can be used as a biomarker of tumor ...
Identifying subcellular localization of microRNAs (miRNAs) is essential for comprehensive understanding of cellular function and has significant implications for drug design. In the past, several ...
After 4 and 7 days, cell viability was measured using the MTT assay. Biodentine and MTA were also prepared and packed into standardized bovine dentin disks and divided into three groups according to ...
In MTT assay, a yellow tetrazole of tetrazolium salt is converted to a purple formazan product by the NADPH-dependent oxidoreductase activity of active cells of the mitochondrial reaction center, ...
针对这一科学难题,罗马大学的研究团队开发了升级版算法MIRROR2(MIRna RegulatiOn-level diffeRential networks),通过整合miRNA-mRNA双层网络分析,揭示了三种癌症(结肠腺癌COAD、肝细胞癌HCC和低级别胶质瘤LGG)中性别二态性的分子机制。
Although diverse cell penetrating motifs not only from naturally occurring proteins but also from synthetic peptides have been discovered and developed, the selectivity of cargo delivery connected to ...
A novel, portable assay can deliver TB diagnoses in under an hour at low cost using samples of blood, saliva, or sputum, according to a recent study.
Despite the fact that thousands of perfluoroalkyl and polyfluoroalkyl substances (PFASs) are in widespread commercial use, analytical methods capable of quantifying them in environmental samples are ...
The in vitro cytotoxicity of iridium (III) carbene complexes against a panel of human cancer cell lines was analyzed by MTT assay (Table 1). Complexes 1a–1e exhibited potent in vitro cytotoxicity ...