资讯

生物通8 年
Western Blot实验中如何选择合理的均一化方法[创新技巧] - 生物通
在Western Blot实验中，用来进行均一化的内参是否合适，是否可以作为绝对定量的均一化的标准？是需要研究者首先在心中打个问号的。那么如何确定选择的内参是否合适？——LI-COR 提供的 Odyssey® Loading Indicators（OLI）可以帮助您解决这个问题。
生物通8 年
Western Blot一定要用看家蛋白做内参？NO！还可以是总蛋白
期刊杂志对Western Blot文章发表的要求越来越严，这些要求包括如何确保Western Blot的重复性、定量的准确性、均一化方法的选择等。Nature、JCB、JBC等杂志明确规定Western Blot进行均一化时，要求选择不受实验条件影响的方法；JBC甚至还建议优先选择总蛋白进行均一化。
Nature7 年
Human adipose tissue protein analyses using capillary western blot technology
A capillary western blot (Wes) technology has recently been validated for analyses of cell culture lysate proteins, but whether it is reliable for human tissue proteins is unknown. We compared ...
The Scientist1 年
Western Blot Protocol, Troubleshooting, and Applications
Western blot sample preparation: Protein extraction and quantification. Sample preparation is a crucial first step in performing an accurate western blot. 4 After extracting total protein from the ...
technologynetworks3 年
Western Blot Procedures, Analysis and Purpose
A western blot, sometimes called a protein immunoblot, is an antibody-based technique used to detect the presence, size and abundance of specific proteins within a sample. The technique was developed ...
technologynetworks3 年
Western Blot Procedures, Analysis and Purpose
A western blot, sometimes called a protein immunoblot, is an antibody-based technique used to detect the presence, size and abundance of specific proteins within a sample. The technique was developed ...
Science Daily9 年
Genome engineering of quantifiable protein tags: Western blot on the way down? - ScienceDaily
Genome engineering of quantifiable protein tags: Western blot on the way down?. ScienceDaily. Retrieved June 2, 2025 from www.sciencedaily.com / releases / 2016 / 06 / 160606082343.htm.
GEN11 年
Making Western Blot Quantification More Reliable
Aldrin V. Gomes, Ph.D. Ning Liu Ph.D. A closer examination of housekeeping and other normalization techniques. In recent years, the scientific community has questioned the reliability of Western ...
FierceBiotech10 年
Western Blot CRO Launches Automated WES Protein Analysis and Protein Expression Services
AUSTIN, Texas, Sept. 3, 2014 /PRNewswire/ -- Western Blot Service CRO, a biology contract research organization based in Austin, Texas, announced today that it is consolidating its years of expert ...
BioTechniques6 年
Western Blot Normalization: Safer Alternative to Housekeeping Proteins - BioTechniques
Western blot normalization is used to determine differences in the levels of a target protein under specific experimental conditions, tissues, time points or developmental stages. Scientists perform ...
The Scientist8 年
ProteinSimple: FluorChem Western Blot Imaging Systems
Meet the trusted FluorChem TM Western blot imaging systems. Simply load your sample, choose a protocol, and hit expose. Load less protein, use smaller amounts of your precious antibodies, and never ...