目前，大肠杆菌也是常用的疫苗表达系统，它和酵母各有优势。大肠杆菌生长快、成本低，能实现高蛋白产量，但无法进行复杂的翻译后修饰，适合生产结构简单的抗原；而酵母能生产结构复杂的重组蛋白，且无内毒素污染，尤其适合生产 VLPs 类疫苗，比如乙肝、HPV 疫苗。选择哪种系统，主要取决于目标疫苗的蛋白特性。

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