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核移植胚胎重编程的细胞特异性差异及关键影响因素探究
二、研究方法 实验动物与胚胎处理:使用非洲爪蟾(Xenopus laevis)进行实验,其饲养遵循德国动物福利法。收集非洲爪蟾的卵子,进行体外受精(IVF)。从神经胚阶段胚胎的内胚层组织分离供体细胞,移植到去核卵子中生成 NT 胚胎。根据 Faber 和 Nieuwkoop 的标准 ...
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