The NoShift Assay procedure (Fig. 1) is a colorimetric assay in which the TF of interest first binds to a double-stranded, synthetic, biotinylated oligonucleotide containing the protein-binding ...
1| Pellet ∼10 8 cells at 300g at 4 °C for 10 min in a centrifuge. The nuclei should be kept cold throughout the procedure. Manipulations and transport to the centrifuge should be done on ice.
Quantification of proteins in various aliquots/fractions and phosphatase activity assay will be carried out during this week. Protein assay will require only about 1 hour while enzyme assay may ...
Samples yielding a grade higher than 10 g/t Au were analyzed a second time by fire assay with gravimetric finish at the same laboratory. Mineralized zones containing visible gold were analyzed with ...
The Alinity m MPXV assay is a real-time PCR test using swabs of skin lesions. It is approved under WHO's Emergency Use Listing procedure. EUL is used for vaccines, tests, and medical treatments.