CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China Department of ...

State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry, Nankai University, ...

Another common use of colorimetric assays includes total protein quantification. For example, the BCA assay is one that combines the reduction of Cu2+ into Cu1+ by proteins in an alkaline medium, with ...

Western blot analysis ... manufacturer's protocol as previously described (22). The protein concentrations were measured by BCA protein assay using a commercial kit from Pierce Biotechnology ...

Western blot (WB) analysis was performed to evaluate the protein expression levels of ... AP0059, Bioworld Technology); β-actin (MG3) monoclonal antibody (Source: mouse, Cat. No. A0101, Lablead); BCA ...

Monoclonal antibodies anti-p32 ASFV protein (S-1D8 and S-5C1) were kindly provided by S.Y. Sunwoo. Rabbit antibody anti-p17 ASFV protein was previously generated in our lab (Suárez et al., 2010).

Find out more about how commercial antibody providers are sharing the responsibility for antibody validation, with Bethyl Laboratories’ solution for a practical, high-throughput method for validating ...

Upon detecting endoplasmic reticulum (ER) stress, the unfolded protein response (UPR) orchestrates adaptive cellular changes to reestablish homeostasis. If stress resolution fails, the UPR commits the ...

This raises the question of whether lipodystrophy and obesity share some common mechanisms that cause fat cells to trigger insulin resistance. One possible player in such mechanisms is a protein ...