Extracted proteins were quantified using the Bradford protein assay. Denatured proteins (25 µg/lane ... and control oligonucleotides, according to the protocol previously defined. Non-transfected ...

In total, all two-component binding reactions, which have a variety of applications in the field of drug design (protein-ligand interactions), mechanisms of membrane-associated proteins ...

Protein assay was done by the method of Bradford. 11 After rats were decapitated ... those mesenteric arteries were embedded in paraffin and cut into 5-μm transverse sections and stained with orcein.

The main steps in a western blot protocol are as follows. Sample preparation ... 4 After extracting total protein from the cells or tissue, researchers must measure the concentration of protein in ...

Hepatitis B virus (HBV) is an important but difficult to study human pathogen. Most basics of the hepadnaviral life-cycle were unraveled using duck HBV (DHBV) as a model although DHBV has a capsid ...

This protocol paper describes how to assign a purity grade and to subsequently titrate ... with a detection limit <50 ng/μl (an order of magnitude higher resolution than that of the Bradford protein ...

The proteostasis network has evolved to support protein folding under normal conditions and to expand this capacity in response to proteotoxic stresses. Nevertheless, many pathogenic states are ...