The assay was performed three times in triplicate. Flow Cytometry Analysis of Cell Cycle and Apoptosis Flow cytometry analysis of cell cycle and apoptosis was performed as described previously by us ...

L3000015, Thermo Fisher Scientific). A total of 3 d post-transfection, cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay (cat no. G7570; Promega) according to the ...

For the B6 macrophage cell line, viability was analyzed using the MTT-based CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). Absorption at 490 nm was detected ... Cell were ...

Metastasis depends upon cancer cell growth and survival within the metastatic niche. Tumors which remodel their glycocalyces, by overexpressing bulky glycoproteins like mucins, exhibit a higher ...

Here we performed a proteomic screen for novel HEG1 interactors and report that HEG1 binds directly to Rasip1. Rasip1 localizes to forming endothelial cell (EC) cell-cell junctions and silencing HEG1 ...