Nitrocellulose membrane binding assay. To compare the binding of APP to soluble and fibrillar Aβ, we used a dot-blot binding assay. Soluble or fibrillar Aβ (4 μg) or the vehicle control (2 μl ...

MTS assay (G3580, Promega) was performed following the manufacturer’s instructions. Absorbance was measured at 490 nm and 630 nm using a SpectraMax i3 plate reader instrument (Molecular Devices LLC).

We evaluated whether the killing ability of WFA is selective, and we explored its mechanism against oral cancer cells. An MTS tetrazolium cell proliferation assay confirmed that WFA selectively killed ...

A complex of NuMA and dynein/dynactin is required for robust spindle orientation in a number of cell types. Previous research proposed that cortical dynein/dynactin was sufficient to generate forces ...

Microtubules (MTs) and actin filaments (F-actin) function cooperatively to regulate plant cell ... Protein concentration was determined by a Bio-Rad protein assay kit. Protein samples of 3 μg were ...