Researchers from several institutions in Brazil developed an enhanced protocol for extracting and analyzing proteins from human skin, combining chemical and mechanical lysis, SP3 sample preparation, ...

Abstract The aim of this study was to extract sheep abomasum protein concentrates (SAPC) by ultrasound-assisted extraction (UAE) and to investigate the properties of SAPC. Response surface methodology ...

One of the central problems in computational biology is to identify the protein function in an automated and high-throughput fashion. A key step in this process is to predict subcellular compartment ...

387 pages : 24 cm "This book describes the mechanisms and experimental procedures for techniques used in protein extraction, purification, and characterization, ranging from classic to cutting-edge." ...

Data on apoptosis, transfection efficiency, cell-surface marker expression, intracellular protein expression, calcium flux and many other properties can be measured. Cells exhibiting specific ...

We have embarked on a detailed investigation of the RJ proteome, using a modified protein extraction protocol and two complementary proteomics approaches, one- and two-dimensional gel electrophoresis ...

Supplementary Table ST4. List of protein groups and peptides identified in each digestion protocol in the E. coli samples with spiked-in control proteins. Supplementary Table ST5. Pearson and Spearman ...

The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth and are marketed ...

The tumor suppressor p53 is a transcription factor that controls the expression of hundreds of genes. Emerging evidence suggests that the p53-induced RNA-binding protein ZMAT3 is a key splicing ...

This important study looks into the effect of exogenous CoA on the response of TLR4-activated macrophages. Specifically, CoA enhances the LPS response by examining metabolomics, 13C tracing, and ...