That might suggest that the high-affinity indicator is strongly saturated, which raises concerns about whether that is impacting ... (2) The use of calcium indicators with very different affinities ...

Currently fasthttp is successfully used by VertaMedia in a production serving up to 200K rps from more than 1.5M concurrent keep-alive connections per physical server.

Buffers and donor or acceptor-coupled sensors were obtained from Revvity ... Anti-human Fc (AHC) biosensors (ForteBio) were soaked for 10 min in PBS, and saturated 15 sec in 1 mM biocytin. Sensors ...