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生物通
1月
固氮基因表达调控的分子机制:NifL-NifA复合物结构解析与工程应用
这篇综述通过冷冻电镜(cryo-EM)和AlphaFold3建模揭示了Azotobacter vinelandii中NifL-NifA复合物的三维结构,阐明了氮固定(nif)基因表达的反激活(anti-activation)分子机制。研究确定了NifL与NifA的相互作用界面,发现氧化态NifL通过其GHKL和DH结构域结合NifA的AAA+ ATPase结构域,阻止 ...
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