SOLVED: A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation:0.0182x 0.007where A is the corrected absorbance of the solution and x is the quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.285. A blank solution has an absorbance of 0.072.Quantity of protein: 10.65 μg

A Calibration curve based on n=10 known points was used to measure the protein in an unknown. The results were protein = 15.22 /- 0.46 micrograms, where the standard deviation is σ = 0.46 micrograms. Find the 90% and 99% confidence intervals for protein in the unknown. The book gives the answer of 15.22 /- 0.86 for the 90% CI and 15.22 /- 1.5 for the 99% CI.

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